Efficacy of Entomopathogenic Fungi, Paecilomyces fumosoroseus, Beauvena bassiana and Metarhizium anisopliae var. majus Against Crocidolomia binotalis (Lepidoptera; Pyralidae)
نویسنده
چکیده
Bioasai di makmal tiga pencilan tempatan entomopatogen hyphomycete ke atas ulat hati kubis, Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae) dan efikasi lapangan beberapa persediaan formulasi Paecilomyces fumosoroseus (Wise) Brown & Smith telah dinilai. Bioasai dos-kernatian menunjukkan kesemua pencilan berupaya menyebabkan maut ke atas larva instar kedua. Kebanyakan larva menjadi moribund dalam masa dua hari selepas rawatan. Pendedahan kepada dos mulai 2 x 1(jl konidia mLl hingga 2 x 107 konidia mLl mengakibatkan purata kernatian larva dari 10.3 hingga 100%. Pada kepekatan melebihi 2 x lOS konidia mt1 kematian larva adalah melebihi 80%, dan 100% kernatian telah didapati pada 2 x 107 konidia mLl bagi ketiga-tiga spesies kulat. Perhubungan signifikan (P<0.005) telah diperolehi di antara log dos dan kernatian probit bagi kesemua pencilan. Nilai EC50 bagi P. fumosoroseus telah dianggarkan pada 1.926 x 1rY konidia mL-1, dan didapati lebih rendah dan signifikan daripada Beauveria bassiana (Bals) Yuill pada 5.038 x 1rY konidia mLl. Nilai EC50 bagi Metarhizium anisopliae var. majus (Metsch) Sorokin adalah yang tertinggi dan signifikan pada 2.0 x 1rr konidia mLl. Pencilan yang terbaik sekali ialah P. fumosoroseus dengan LT50 hampir setengah hari lebih singkat pada dos setanding 2 x 10 konidia mLl, dengan nilai kecerunan 4.656 berbanding 4.356 bagi B. bassiana dan 4.193 bagi M. anisopliae var. majus. Pada rawatan lapangan, tanaman telah disembur dengan ampaian mengandungi 10 konidia mLl. Purata peratus kematian bagi kesemua rawatan didapati melebihi 70 % dan lebih signifikan (P<O. 05) daripada kawalan. Konidia yang diampai dalam minyak kelapa sawit Vesawit® telah memberi keputusan yang paling menggalakkan ke atas ulat hati kubis. ABSTRACT
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